Logo-japid
Submitted: 01 Jan 2012
Accepted: 23 Mar 2012
ePublished: 29 Sep 2018
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

J Periodontol Implant Dent. 2012;4(2): 29-33.
  Abstract View: 143
  PDF Download: 90

Research Article

The Micronucleus Test to Evaluate Cytogenetic Damage in Patients with Periodontitis

Haritha Avula 1* ORCID logo, Ashank Mishra 1, Ruchi Pandey 1, Murali Krishna 1, Butchi babu Kalakonda 1, Jayakumar Avula 1

1 Department of Periodontics, Sri Sai College of Dental Surgery, Vikarabad, Andhra Pradesh, India
*Corresponding Author; E-mail: harithaavula@gmail.com

Abstract

Background and aims. The role of genetic factors and oxidative damage in the etiopathogenesis of periodontal disease is well documented in the literature. The micronucleus test is a sensitive method that indicates DNA damage. The aim of the present study was to investigate the micronuclei frequency in different forms of periodontitis in comparison with healthy controls.

Materials and methods. Micronuclei frequency was analyzed in the peripheral lymphocytes of 10 patients with chronic periodontitis (CP), 10 patients with generalized aggressive periodontitis (GAP) and 10 healthy controls. Various clinical parameters like the probing depth, clinical attachment level, and percentages of sites with bleeding and plaque were recorded. After the slides were processed, they were stained with 5% Geimsa solution and 1000 cells per sample were counted for the presence of micronuclei.

Results. The mean micronuclei observed in the control group, CP group, and GAP group were 9.8, 10.1 and 9.9, respectively. The differences between the groups were not significant (P=0.978).

Conclusion. The results indicated that the cytogenetic damages in the periodontitis groups were not different from those in the control group. The role of cytogenetic damage in peripheral blood cells may have a limited prognostic value in the etiopathogenesis of periodontal tissues and further studies are necessary to assess cytogenetic damage in periodontal tissues to clarify local tissue destruction in periodontal disease. 

Keywords: Micronucleus test, aggressive periodontitis, chronic periodontitis, cytogenetic damage
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 143

Your browser does not support the canvas element.


PDF Download: 90

Your browser does not support the canvas element.