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Submitted: 09 Feb 2016
Accepted: 05 Apr 2016
ePublished: 09 Oct 2016
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J Periodontol Implant Dent. 2016;8(1): 24-32.
doi: 10.15171/jpid.2016.005
  Abstract View: 205
  PDF Download: 232

Research Article

Assessment of Gingival Fibroblast Attachment to Root Surfaces Restored with Three Different Dental Materials: An in Vitro Study

Reza Amid 1, Maryam Torshabi 2, Kasra Tabari 3, Mahdi Kadkhodazadeh 4, Sarah Eslami 5*, Mitra Ghazizadeh Ahsaie 6

1 Associate Professor, Department of Periodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Assistant Professor, Department of Dental Biomaterial, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Assistant Professor, Department of Operative Dentistry, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Associate Professor, Department of Periodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Postgraduate Student, Dental School, Islamic Azad University, Tehran, Iran
6 Dental Student, Gifted and Talented Dental Students Division, Students’ Research Committee, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
*Corresponding Author; E-mail: eslami_sarah@yahoo.com

Abstract

Background and aims. The aim of this study was to compare the biocompatibility of calcium-enriched mixture cement (CEM), composite resin and nano-particled mineral trioxide aggregate (NP-MTA) using human gingival fibroblasts.

Materials and methods. A comparative in vitro cell culture study was carried out using 60 single-rooted teeth which were assigned to the following four groups: 1) untreated healthy group (control); 2) restored with composite resin; 3) CEM cement; 4) NP-MTA. The MTT assay was used to measure the viability of fibroblasts attached to each specimen and scanning electron microscopy (SEM) was used for describing cell morphology.

Results. After 24 hours of incubation, the survival rates for composite resin and NP-MTA were 74.1% and 76.9%, respectively, which were significantly lower than the value in the control group, while both were equally biocompatible. No statistically significant difference was found between the control group and CEM cement samples (94.3%). After 3 days of incubation, some increases in the viability of fibroblasts were detected in the composite resin and NP-MTA groups, with their survival rates being 89% and 93%, respectively. Conversely, in the CEM cement group, the survival rate decreased to 80.7%, which was significantly lower than that in the control group (P = 0.0001).

Conclusion. The results of in vitro tests indicated that on days 1, 3 and 5 after incubation, composite resin, CEM cement and NP-MTA exhibited acceptable biocompatibility, provided they were allowed to set for 24 hours before exposure to the cells.  

Keywords: Gingival fibroblast attachment, Mineral trioxide aggregate, Calcium-enriched cement
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