Abstract
Abstract
Background. Suture materials in the oral cavity can promote bacterial adhesion and contamination. While antimicrobial-coated sutures are effective, their use is limited by cost, availability, and toxicity. Platelet rich Fibrin (PRF) derivatives have shown antimicrobial activity against periodontal pathogens. This study compared the antimicrobial efficacy of sutures soaked in saline, i-PRF, and PRF lysate
Methods. An in-vitro study was used to determine the minimum inhibitory concentration (MIC) of sutures soaked in saline, i-PRF, and PRF lysate. Sutures were tested against Streptococcus mutans, Prevotella intermedia, and Porphyromonas gingivalis by measuring inhibition zones. Nonabsorbable 3-0 black silk sutures were cut and soaked for 10 minutes in saline (Group A), i-PRF (Group B), or PRF lysate (Group C), then incubated anaerobically at 37°C for 24 hours. Additionally, samples from all three groups were incubated at 37°C in 5 ml of saliva from patients with chronic periodontitis, and colony-forming units (CFUs) were assessed on days 1, 3, 5, and 7.
Results. Sutures soaked with i-PRF and PRF lysate demonstrated a statistically significant increase in the zone of inhibition and a reduction in CFU against Streptococcus mutans, Prevotella intermedia, and Porphyromonas gingivalis. SEM (Scanning Electron Microscopy) analysis showed fibrin network on sutures soaked in i-PRF and PRF lysate. Conclusion. The antimicrobial efficacy of i-PRF and PRF lysate treated sutures oral pathogens appears promising. These biologically enhanced sutures may serve as effective alternatives for conventional antimicrobial sutures.